The smart Trick of HPLC columns That No One is Discussing
If your mobile period’s pH is sufficiently acidic, the solutes are present as neutral weak acids which might be a lot more soluble in the stationary period and get longer to elute. Since the weak acid solutes don't have equivalent p-hydroxybenzoic acid (PH) over a nonpolar C18 column topic into a most analysis time of six min. The shaded location